Read e-book online Advanced Methods in Cellular Immunology PDF

By Rafael Fernandez-Botran

ISBN-10: 0849321255

ISBN-13: 9780849321252

ISBN-10: 1420039237

ISBN-13: 9781420039238

Immunologists in addition investigators in different disciplines may well frequently use protocols related to the isolation, cultures and characterization of alternative different types of leukocytes. complex equipment in mobile Immunology is a suite of suggestions in an easy-to-use format.Each bankruptcy offers readers with similar software details, a step by step description of the method, substitute options, pertinent references, and data approximately advertisement resources for fabrics and regents. as well as leukocytes, the authors advisor readers throughout the techniques of telephone tradition in addition to irritation and autoimmunity in a number of animal models.Covering themes reminiscent of PCR and Apoptosis, this publication will serve a consultant to prevalent tactics in mobile immunology whereas using either human and murine types.

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Reagents, 130, 149, 1990. 4. Myers, M. , Direct measurement of cell numbers in microtiter plate cultures using the fluorescent dye SYBR green I, J. Immunol. , 212, 99, 1998. 5. , Bartal, A. , An enzymelinked immunosorbent assay (ELISA) for the detection of monoclonal antibodies recognizing antigens expressed on viable cells, J. Immunol. Reagents, 58, 301, 1983. 6. , DNA staining for fluorescence and laser confocal microscopy, J. Histochem. , 45, 49, 1997. 7. Denizot, F. , Rapid colorimetric assay for cell growth and survival.

60 B. 62 C. 64 D. 64 V. 65 A. 66 VI. 67 VII. 68 VIII. 69 IX.

Econo-SafeTM, Research Products International) • Liquid scintillation counter Protocol 1. 2 ml. , dilution) should be tested in triplicate. Include a background proliferation control in which the cells are cultured in medium alone. Incubate at 37°C in a CO2 incubator for the appropriate length of time. 2. Dilute the [3H-methyl] thymidine stock solution with culture medium to a concentration of 50 µCi/ml. With the aid of a multichannel pipette, add 20 µl of the diluted solution to each well. This gives a final concentration of 1 µCi per well.

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Advanced Methods in Cellular Immunology by Rafael Fernandez-Botran

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