By Rodolfo Paoletti, Dr. David Kritchevsky
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Extra resources for Advances in lipid research. Vol.10
Pollard et al. (1969) obtained very different micrographs after using essentially the same negative staining technique. Electron microscopy of LDL by these authors resulted in the images shown in Fig. 13F. The LDL in this micrograph are very irregular in shape and variable in size. The broad range in apparent size suggests that some of the particles may be partially buried in and obscured by the stain. Figure 14 is a diagrammatic sketch of the top and side views of the LDL image as observed in our micrographs.
1970). /. Cell Biol. 45, 649. Van Bruggen, E. F. , Wiebenga, E. , and Gruber, M. (1962). /. Mol. Biol. 4, 1. Zilversmit, D. B. (1965). /. Clin. Invest. 44, 1610. Zilversmit, D. B. ( 1968). /. Lipid Res. 9, 180. Zilversmit, D. B. (1969). In "Structural and Functional Aspects of Lipoproteins in Living Systems" ( E . Tria and A. M. ), pp. 329-368. Academic Press, New York. Employment of Lipids in the Measurement and Modification of Cellular, Humoral, and Immune Responses NICHOLAS R. Di LUZIO Department of Physiology, Tulane University School of New Orleans, Louisiana Medicine, I.
Robert Hamilton. ) IV. Concluding Remarks The various techniques of electron microscopy can be exceedingly useful for studying the fine structure of the various serum lipoprotein classes. In this review we have outlined the capabilities of techniques such as fixation and shadow-casting, freeze-etching, and negative staining. The advantages and limitations of the methods in their applicability to studies on lipoproteins have been discussed. Electron microscopy has proved particularly useful for investigating the structure of lipoproteins in certain disease states.
Advances in lipid research. Vol.10 by Rodolfo Paoletti, Dr. David Kritchevsky